Tomasz Brudek

​​Senior researcher

Ph.D., cand.scient.

​Contact: +45 38635600 / tomasz.brudek@regionh.dk

Alpha-Synuclein is a presynaptic neuronal protein that is linked genetically and neuropathologically to Parkinson’s disease (PD), Multiple System Atrophy (MSA) and Dementia with Lewy bodies (DLBs). α-Synuclein may contribute to PD and MSA pathogenesis in a number of ways. I have focused my research work on investing the role of different α-synuclein species such as monomers (isoforms, truncated variants), oligomers, fibrils, and α-synuclein post-translational modifications in these two synucleinopathies.

Moreover, a growing body of evidence from clinical studies and preclinical animal models emphasize a role of the immune system in the development of PD and MSA. I am concentrating my research on anti-α-synuclein naturally occurring autoantibodies (NAbs) as therapeutic targets and clinical biomarkers. NAbs make up two-thirds of the human antibody pool and may be involved in many functions such as regulatory and protective actions. NAbs in the central nervous system has been implicated in maintaining homeostasis by removing cell debris and are suggested to prevent inflammation by binding and neutralizing cytokines.
My research focus concentrates not only on synucleinopathies but also other proteinopathies including Alzheimer’s disease (AD) and Amyotrophic Lateral Sclerosis (ALS).​

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In near future we plan to expand the molecular studies from tissue specific to cell specific by combining UV-laser microdissection- and quantitative RT-PCR techniques with a suitable PCR assays.

​Publications​​ the las 5 years

Brudek T.  Inflammatory Bowel Diseases and Parkinson’s Disease
Journal of Parkinson's Disease, pp. 1-14, 2019

Folke J, Rydbirk R,  Løkkegaard A, Salvesen L,  Hejl AM,  Starhof C, Bech S, Winge K, Christensen S, Østergaard Pedersen L, Aznar S, Pakkenberg B,  Brudek T. Distinct Autoimmune Anti-α-Synuclein Antibody Patterns in Multiple System Atrophy and Parkinson’s Disease. Frontiers in Immunology 2019, 2253

Villumsen M, Aznar S, Pakkenberg B, Brudek T, Jess T. Authors' response: Association between IBD and Parkinson's disease: seek and you shall find? Gut. 2019 Sep;68(9):1722.

Rydbirk R, Elfving B, Folke J, Pakkenberg B, Winge K, Brudek T, Aznar S. Increased prefrontal cortex interleukin-2 protein levels and shift in the peripheral T cell population in progressive supranuclear palsy patients. Sci Rep. 2019 May 23;9(1):7781. 

Folke J, Pakkenberg B, Brudek T. Impaired Wnt Signaling in the Prefrontal Cortex of Alzheimer's Disease. Mol Neurobiol. 2019 Feb;56(2):873-891. 

Villumsen M, Aznar S, Pakkenberg B, Jess T, Brudek T. Inflammatory bowel disease increases the risk of Parkinson's disease: a Danish nationwide cohort study 1977-2014.Gut. 2019 Jan;68(1):18-24. 

Betzer C, Lassen LB, Olsen A, Kofoed RH, Reimer L, Gregersen E, Zheng J, Calì T, Gai WP, Chen T, Moeller A, Brini M, Fu Y, Halliday G, Brudek T, Aznar S, Pakkenberg B, Andersen JP, Jensen PH. Alpha-synuclein aggregates activate calcium pump SERCA leading to calcium dysregulation. EMBO Rep. 2018 May;19(5). pii: e44617. 

Fomsgaard L, Moreno JL, de la Fuente Revenga M, Brudek T, Adamsen D,
Rio-Alamos C, Saunders J, Klein AB, Oliveras I, Cañete T, Blazquez G, Tobeña A,
Fernandez-Teruel A, Gonzalez-Maeso J, Aznar S. Differences in 5-HT2A and mGlu2
Receptor Expression Levels and Repressive Epigenetic Modifications at the 5-HT2A Promoter Region in the Roman Low- (RLA-I) and High- (RHA-I) Avoidance Rat
Strains. Mol Neurobiol. 2018 Mar;55(3):1998-2012.

Rydbirk R, Elfving B, Andersen MD, Langbøl MA, Folke J, Winge K, Pakkenberg B, Brudek T, Aznar S. Cytokine profiling in the prefrontal cortex of Parkinson's Disease and Multiple System Atrophy patients. Neurobiol Dis. 2017 Oct;106:269-278. 

Brudek T, Winge K, Folke J, Christensen S, Fog K, Pakkenberg B, Pedersen LØ.
Autoimmune antibody decline in Parkinson's disease and Multiple System Atrophy; a step towards immunotherapeutic strategies. Mol Neurodegener. 2017 Jun 7;12(1):44.

Nykjaer CH, Brudek T, Salvesen L, Pakkenberg B. Changes in the cell population
in brain white matter in multiple system atrophy. Mov Disord. 2017 Apr 10. 

Russ K, Flores J, Brudek T, Doudet DJ. Differential behavioral outcomes
following neonatal versus fetal human retinal pigment epithelial cell striatal
implants in parkinsonian rats. J Neural Transm (Vienna). 2017 Apr;124(4):455-462.

Rydbirk R, Folke J, Winge K, Aznar S, Pakkenberg B, Brudek T. Assessment of
brain reference genes for RT-qPCR studies in neurodegenerative diseases. Sci Rep.2016 Nov 17;6:37116. 

Rasmussen NB, Olesen MV, Brudek T, Plenge P, Klein AB, Westin JE, Fog K,
Wörtwein G, Aznar S. 5-HT2A Receptor Binding in the Frontal Cortex of Parkinson's
Disease Patients and Alpha-Synuclein Overexpressing Mice: A Postmortem Study.
Parkinsons Dis. 2016;2016:3682936. 

Russ K, Flores J, Brudek T, Doudet D. Neonatal Human Retinal Pigment Epithelial Cells Secrete Limited Trophic Factors in Vitro and in Vivo Following Striatal Implantation in Parkinsonian Rats. Journal of Neural Transmission, Nov 6 2015.

Brudek T, Winge K, Rasmussen NB, Bahl JM, Tanassi J, Agander TK, Hyde TM,
Pakkenberg B. Altered α-synuclein, parkin, and synphilin isoform levels in
multiple system atrophy brains. J Neurochem. 2016 Jan;136(1):172-85. 

Salvesen L, Winge K, Brudek T, Agander TK, Løkkegaard A, Pakkenberg B. Neocortical Neuronal Loss in Patients with Multiple System Atrophy: A Stereological Study. Cerebral Cortex 2015 Oct 13, Epub ahead of print 

Salvesen L, Ullerup BH, Sunay FB, Brudek T, Løkkegaard A, Agander TK, Winge K, Pakkenberg B. Changes in total cell numbers of the basal ganglia in patients with multiple system atrophy - A stereological study. Neurobiol Dis. Neurobiol Dis. 2015 Feb;74:104-13.

Vlassaks E, Brudek T, Pakkenberg B, Gavilanes AW. Cerebellar cytokine expression in a rat model for fetal asphyctic preconditioning and perinatal asphyxia. Cerebellum. 2014 Aug;13(4):471-8.
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